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| Registros recuperados: 174 | |
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| Kuchiishi,S.S.; Carvalho,L.F.O.S.; Piffer,I.A.; Kich,J.D.; Ramenzoni,M.L.F.. |
| Três ELISAs polivalentes baseados em lipopolissacarídeos de cadeia longa (LPS-CL) foram estabelecidos para detectar anticorpos para todos os sorotipos prevalentes de Actinobacillus pleuropneumoniae. Foram testadas amostras provenientes do banco de soros de suínos experimentalmente inoculados com todos os sorotipos de A. pleuropneumoniae. Os ELISAs foram sensíveis à detecção de anticorpos contra todos os LPS-CL. Foram observadas reações cruzadas no ELISA polivalente produzido com os sorotipos 1 e 5, com anti-soros específicos para os sorotipos 9 e 11, pois os sorotipos 1, 9 e 11 apresentaram antígenos somáticos comuns. No polivalente com os sorotipos 2, 3 e 7, observaram-se reações com anti-soros dos sorotipos 4, 6 e 8, devido à presença de antígenos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ELISA; Pleuropneumonia; Actinobacillus pleuropneumoniae; Sorologia; LPS. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200016 |
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| Nickel,Osmar; Targon,Maria L.P.N.; Fajardo,Thor V.M.; Machado,Marcos A.; Trivilin,Ana P.. |
| The coat protein gene of Apple stem grooving virus (ASGV) was amplified by RT-PCR, cloned, sequenced and subcloned in the expression vector pMal-c2. This plasmid was used to transform Escherichia coli BL21c+ competent cells. The ASGV coat protein (cp) was expressed as a fusion protein containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP fusion protein was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fusion protein gave specific reactions to ASGV from infected apple (Malus domestica) cv. Fuji Irradiada and Chenopodium quinoa at dilutions of up to 1:1,000 and 1:2,000, respectively, in plate trapped ELISA. The ASGVcp/MBP... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Recombinant antigen; Latent virus; ELISA; Malus domestica. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000500017 |
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| Delgado,Irma; Alfonso,A; Martínez,Nadia; Abeledo,María Antonia; Rodríguez,Majela; Barrera,Maritza. |
| El objetivo de este trabajo fue realizar un estudio serológico a varios rebaños bovinos adultos seleccionados por su potencialidad para el comercio. Un total de 597 muestras de suero de bovinos adultos sanos, procedentes de Pinar del Río, La Habana, Ciudad Habana y Villa Clara, fueron analizadas a través de un ELISA indirecto. El 25,9% de las muestras estudiadas resultaron positivas a anticuerpos contra el VLB. Los resultados obtenidos coinciden con reportes anteriores para la región, donde se refiere una alta prevalencia de anticuerpos a este virus. El estudio permitió obtener información sobre la presencia de anticuerpos al VLB en bovinos adultos. Estos resultados sugieren la necesidad de realizar una encuesta para determinar la seroprevalencia en... |
| Tipo: Journal article |
Palavras-chave: Virus leucosis bovina enzoótica; ELISA; Leucosis; Anticuerpos; Bovinos. |
| Ano: 2009 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000100005 |
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| Alves,L.A.O.; Teixeira,M.F.S.; Pinheiro,A.A.; Pinheiro,R.R.; Dias,R.P.; Brito,R.L.L.; Lopes Júnior,C.A.F.; Bezerra Júnior,R.Q.; Azevedo,D.A.A.. |
| Este estudo teve como objetivo produzir um antígeno (Ag) a partir de cultura de células de membrana sinovial caprina (MSC) infectadas com o vírus de artrite encefalite caprina (CAEV), pela técnica de microfiltração seriada, substituindo a ultracentrifugação em colchão de sacarose (UCCS) para utilização em ELISA indireto (ELISA-i). Amostras de 188 soros caprinos, que previamente foram testados pelo Western blot (WB) com Ag UCCS, foram submetidas à análise pelo ELISA-i com o novo antígeno produzido, que mostrou concordância de 92% em relação ao antígeno UCCS. A sensibilidade e a especificidade do ELISA em relação ao WB foram de 95,6% e 88,5%, respectivamente. A nova técnica, criada a partir de microfiltrações, mostrou-se efetiva e de baixo custo para o... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caprino; CAEV; ELISA; Lentivírus de pequenos ruminantes; Western blot. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400021 |
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| Leal Júnior,Gildemberg A; Duarte,Keila M. R; Albuquerque,Paulo S. B; Tavares,Flávio C. A; Figueira,Antonio. |
| A obtenção de soros policlonais contra fitopatógenos tem por finalidade a elaboração de imunoensaios que permitam identificar e caracterizar os mesmos com rapidez e a custos baixos. Soros policlonais foram produzidos contra extratos das hifas de isolados de Crinipellis perniciosa, agente causal da vassoura-de-bruxa, coletados de cacaueiro (Theobroma cacao) nos estados do Pará e Bahia para identificação e caracterização de isolados de diversas regiões e hospedeiros. O soro denominado de 1 foi produzido a partir do isolado ESJOH-1 (originário do Pará), e o soro 2 foi produzido com o isolado CP-85 (originário da Bahia). Não houve reação cruzada dos soros contra extratos protéicos das espécies Alternaria solani, Marasmius sp., Oudemansiella canarii e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ELISA; Moniliophthora roreri; Variabilidade; Vassoura-de-bruxa; Cacaueiro; Theobroma. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000500004 |
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| Monte,Leonardo Garcia; Leal,Fernanda Munhoz Dos Anjos; Hartwig,Daiane Drawanz; Vasconcellos,Sílvio Arruda; Brihuega,Bibiana; Dellagostin,Odir Antonio; Hartleben,Cláudia Pinho. |
| The production of recombinant LipL32 protein using Escherichia coli has been used extensively for the development of vaccines and diagnostic tests for leptospirosis. However, E. coli has demonstrated limitations, including low yield and lack of post-translational modifications. In this study, rLipL32 was produced in eukaryotic expression system (Pichia pastoris) and evaluated the antigen by enzyme-linked immunosorbent assay (ELISA). The yield obtained from the culture supernatant reached 270 mg/L and ELISA showed an accuracy of 95.34%. In summary, the production of rLipL32 using P. pastoris did not impair the antigenic characteristics of this antigen and ensured its use for detecting the leptospiral antibodies in swine sera. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pichia pastoris; LipL32; ELISA; Leptospirosis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300007 |
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| Chaves,Daniel Gonçalves; Rodrigues,Cibele Velloso; Ferreira,Wanderley Almeida; Siqueira,Pollyana Fantini Miranda; Duarte,Clara Guerra; Carneiro-Proietti,Anna Bárbara de Freitas; Santoro,Marcelo Matos. |
| In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Factor IX; Hemophilia B; HPLC; Purification; Antibodies; ELISA. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010 |
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| Litaker, R. Wayne; Stewart, Thomas N.; Eberhart, Bich-thuy L.; Wekell, John C.; Trainer, Vera L.; Kudela, Raphael M.; Miller, Peter E.; Roberts, Alice; Hertz, Cassandra; Johnson, Tyler A.; Frankfurter, Greg; Smith, G. Jason; Schnetzer, Astrid; Schumacker, Joe; Bastian, Jonnette L.; Odell, Anthony; Gentien, Patrick; Le Gal, Dominique; Hardison, I. Ransom; Tester, Patricia A.. |
| Domoic acid (DA) is a potent toxin produced by bloom-forming phytoplankton in the genus which is responsible for causing amnesic shellfish poisoning (ASP) in humans. ASP symptoms include vomiting. diarrhea, and in more severe cases confusion. loss of memory. disorientation. and even coma or death. This paper describes the development and validation of a rapid. sensitive. enzyme linked immunosorbent assay test kit for detecting DA using a monoclonal antibody. The assay gives equivalent results to those obtained using standard high performance liquid chromatography. fluorenylmetbox-yearbonyl high performance liquid chromatography, or liquid chromatography-mass spectrometry methods. It has a linear range from 1.1-3 ppb and was used successfully to measure DA... |
| Tipo: Text |
Palavras-chave: Test kit; Razor clams; Scallops; Mussels; ELISA; Domoic acid poisoning; ASP. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6253.pdf |
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| Peron,G.; Fernandes,F.F.; Landgraf,T.N.; Martinez,R.; Panunto-Castelo,A.. |
| Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paracoccidioidomycosis; Heat shock protein; Serodiagnosis; ELISA; Western blotting. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400705 |
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| Leal,Alexandre Trindade; Santurio,Janio Morais; Leal,Adriana Bardemaker Monteiro; Pinto,Alexandre Machado; Griebeler,Josiane; Flores,Eduardo Furtado; Ferreiro,Laerte; Catto,João Batista. |
| O Pythium insidiosum é um fungo zoospórico que se desenvolve em locais alagadiços e que pode infectar humanos e animais, principalmente eqüinos. A infecção natural nesta espécie resulta em pitiose clínica, uma doença granulomatosa de difícil tratamento. Uma das opções terapêuticas é a imunoterapia com antígenos obtidos de culturas do agente. Com o objetivo de avaliar o efeito de adjuvantes na resposta sorológica a antígenos do P. insidiosum, 24 coelhos divididos em 4 grupos foram imunizados com antígeno macerado de micélio (AMM) associado a três adjuvantes. Grupo I: hidróxido de alumínio; grupo II: adjuvante de Freund; grupo III: óleo mineral e grupo IV: água destilada -controle. Os tratamentos foram avaliados a diferentes intervalos, quanto à capacidade... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pythium insidiosum; Imunoterapia; Adjuvantes; Coelhos; ELISA. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000600017 |
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| Breuil, Gilles; Mouchel, Olivier; Fauvel, Christian; Pepin, Jean-francois. |
| Reproduction of nodavirus disease was performed by experimental infection of sea bass eggs during fertilization or at larval stage 4 with 2 genetically distinguishable nodavirus strains (Sb1 and Sb2) isolated from sea bass collected along the Atlantic and Mediterranean French coast. The pathogenicity of the virus strains was assigned after detection of the virus by ELISA and immunohistochemistry (IHC). The Atlantic (Sb1) strain was more pathogenic than the Mediterranean (Sb2) strain during the fertilization step whilst both strains were pathogenic following experimental exposure of 4 d old larvae. Virus lesions developed in the brain 4 to 6 d following experimental exposure. Experimental ELISA proved very sensitive for detecting the nodavirus in Sb1 or Sb2... |
| Tipo: Text |
Palavras-chave: ELISA; Nodavirus; Fish encephalopathy. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-814.pdf |
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| Tumurjav, Buyannemekh; Terkawi, Mohamad Alaa; Zhang, Houshuang; Zhang, Guohong; Jia, Honglin; Goo, Youn-Kyoung; Yamagishi, Junya; Nishikawa, Yoshifumi; Igarashi, Ikuo; Sugimoto, Chihiro; Xuan, Xuenan. |
| Toxoplasma gondii matrix antigen 1 (TgMAG1), known as the 65-kDa protein, which is abundantly expressed in both bradyzoites and tachyzoites, was evaluated as a candidate for the development of a diagnostic reagent for ovine toxoplasmosis. The TgMAG1 gene was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST),and the recombinant TgMAG1 (rTgMAG1) was tested in an enzyme-linked immunosorbent assay (ELISA). The ELISA with rTgMAG1 showed a highly specific reaction with sera from mice experimentally infected with T. gondii but not with the closely related Neospora caninum. The antibodies to TgMAG1 were detectable from the acute to the chronic infectious stages in a mouse model. A total of 175 serum samples collected from sheep... |
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Palavras-chave: ELISA; Mongolia; Sheep; TgMAG1; Toxoplasma gondii. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3018 |
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| Registros recuperados: 174 | |
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